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The Histology of the Common Frog by Rose B. Ankeny, 1887

The Histology of the Common Frog by Rose B. Ankeny Edgar, 1887, Page 5

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3. of carmine and gelatine is most valuable for this purpose. The injecting is done in different ways with different subjects. After injection all the tissues are hardened by placing the specimen in alcohol for a number of hours. Then the particular portion to be examined is embedded in a mixture of parafine and vasaline in whatever position is desired, and cut in very thin sections. In case of the examination for cellular structure the specimen is first hardened in alcohol, then embedded and cut, after which the sections are allowed to remain in a straining
 
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